Cytokeratin cocktail by IHC

Alphabetical Test listing

Cytokeratin cocktail by IHC-12376 - Technical only, 12379 - Technical & interpretation

  
Cytokeratin cocktail by IHC
  
12376 - Technical only, 12379 - Technical & interpretation
  
LAB12376
LAB12379
  
CK cocktail
  

All IHC stains will include a positive control tissue

  
Tissue
  

Submit a formalin-fixed, paraffin-embedded tissue

  

Formalin-fixed, paraffin-embedded (FFPE) tissue block

  

FFPE tissue section mounted on a charged, unstained slide

  

Ambient (preferred)

  
  • Unlabeled/mislabeled block
  • Insufficient tissue
  • Slides broken beyond repair
  
AHL - Immunohistochemistry
  
Mo - Fr
  
1 - 2 days
  

Immunohistochemical staining and microscopic examination

  

If requested, an interpretive report will be provided

  

Specifications

  • Family of 20 different heterogenous proteins 40-70 kD NW
  • All epithelial cells contain cytokeratins, but different types of epithelium contain different keratin profiles
  • Low MW found in simple and glandular epithelium; High MW found in complex stratified epithelium
  • CK cocktails cover the widest range of kD weights

 
Staining pattern

Cytoplasmic Anomalous CK expression:

  • melanomas
  • smooth muscle cells and smooth muscle tumors
  • astrocytes and astro. tumors (glioblastoma multiforme)
  • endothelial cells and angiosarcomas

When a routine CK is ordered, a cocktail of 3 different cytokeratins is automatically used: AE1-AE, CAM5.2 and CK18.


References

  1. Moll et. al.; The Catalog of Human Cytokeratins: Patterns of Expression in Normal Epithelia, Tumors and Cultured Cells; Cell 31:11-24, 1982.
  
88342 - 1st stain
88341 - each additional stain
  
06/19/2017
  
08/26/2019
  
10/02/2020