Ber-EP4 by IHC

Alphabetical Test listing

Ber-EP4 by IHC-12376 - Technical only, 12379 - Technical & interpretation

Ber-EP4 by IHC
12376 - Technical only, 12379 - Technical & interpretation
  • All IHC stains will include a positive control tissue

Ber EP4 can be useful in a panel of immunostains to differentiate between mesothelioma and adenocarcinoma

  • The majority of lung and other adenocarcinomas are reactive with Ber EP4
  • Mesotheliomas are usually negative for Ber EP4. However, 1-11% are reportedly positive for Ber EP4.
    • Only one report to date showed a relatively high rate of positivity (20%) for mesotheliomas with Ber EP4 (see Gaffey et al)
    • A staining pattern of CEA negative, Ber EP4 negative and LeuM1 (CD15) negative is said to be 100% specific for mesothelioma. (See Grove et al.)
    • A staining pattern of Ber EP4 positive, CEA positive and LeuM1 positive is said to be 96.8% specific for adenocarcinoma (See Grove et al)
    • Renal cell carcinoma and breast carcinomas are sometimes negative (25-50% of the time) for Ber EP4 or only very focally positive (see Gaffey et al, Sheibani et al)
    • False positives with Ber EP4 have been reported in cell block material (cytology) in peritoneal fluids from females (endosalpingiosis, endometriosis, and leiomyomata). (See Diaz-Arias et al).
  • Reactivity with Ber EP4

    Normal Tissues:
    All human epithelial cells


100% pulmonary adenocarcinoma
100% pancreas
100% colonic adenocarcinoma
100% gastric adenocarcinoma
100% endometrial
100% thyroid carcinoma
80-100% ovarian adenocarcinoma (mucinous and serous)
88% prostatic adenocarcinoma
up to 75% breast carcinoma (ductal and lobular)
70% anaplastic (CD30+) large cell lymphomas
65% small cell of lung
50% renal cell carcinoma
29% squamous carcinoma of lung
1-20% mesotheliomas





Submit a formalin-fixed, paraffin embedded tissue block


Formalin-fixed, paraffin embedded (FFPE) tissue block


Tissue section mounted on a charged, unstained slide


Ambient (preferred)

  • Unlabeled/mislabeled block
  • Insufficient tissue
  • Slides broken beyond repair
AHL - Immunohistochemistry
Mo - Fr
1 - 2 days

Immunohistochemical staining and microscopic examination


If requested, an interpretive report will be provided



  • Ber EP4 is a cell surface glycoprotein present on most human epithelial cells
  • Ber EP4 reacts with most epithelial tumors and is recognized as an epitope that is different from other epithelial antigens (i.e. keratins or CEA)

 Staining patterns

  • Cell membrane and occasionally diffuse cytoplasmic


  1. Gaffey et al. Am J Surg Pathol, 16(6):593-599, 1992.
  2. Diaz-Arias et al. Diag Cytopath, 19(5):516-521, 1993.
  3. Grove et al. Path Res and Prac, 1990:1044-1055, 1994.
  4. Sheibani, et al. Am J Surg Pathol, 15(8):779-784, 1991.
88342 - 1st stain
88341 - each additional stain