Galactose-1-phosphate in RBCs

Alphabetical Test listing

Galactose-1-phosphate in RBCs-994

  
Galactose-1-phosphate in RBCs
  
994
  
LAB994
  
MSO
  

Use to monitor treatment, response, and compliance with dietary restriction for patients with an established diagnosis of galactosemia

  
EDTA whole blood
  
  
5 mL
  
2.0 mL
  
  • Place tube on wet ice immediately after collection
  • Do not freeze
  

Lavender (EDTA), 4mL

  
Heparin whole blood;Washed, packed red blood cells (EDTA or heparin)
  

Dk green heparin (Li or Na), no gel

 

 

  
Washed, packed RBC's: 3.0 (minimum 0.6) mL
  

Washed, packed RBC's:

  1. Centrifuge whole blood immediately for 10 minutes
  2. Discard the plasma and buffy coat layers
  3. Add cold 0.9 percent saline solution to the red blood cells (about 2 times the volume of cells) and mix gently by inverting the tube
  4. Centrifuge for 10 minutes
  5. Aspirate and discard the supernatant
  6. Repeat the wash (steps 3 through 5) 2 more times
  7. After the third wash and centrifugation, remove the supernatant and a thin layer of the top cells
  8. Transfer washed cells to a Screw-cap polypropylene frozen transport vial/tube - 4mL (LabCorp) labeled as washed cells and freeze
  

Whole blood:

Dk green heparin (Li or Na), no gel

Washed, packed RBC's:

Screw-cap polypropylene frozen transport vial/tube - 4mL (LabCorp)

  

Whole blood:

Refrigerated - 72 hours

Ambient - NO

Frozen - NO

Packed RBC (alternative):

Frozen

 

 

  
  • Whole blood received at room temperature or frozen are unacceptable
  • RBC’s received at room temperature or refrigerated
  
ARUP (0081296) via LabCorp (806520): R-NX
  
Tu
  
2 - 9 days
  

Gas Chromatography-Mass Spectrometry (GC-MS)

  

Galactose-1-phosphate:   0.0-1.0 mg/dL

Galactose-1-phosphate:   0-53 µg/g Hb

Galactose-1-phosphate:  0.00-0.20 µmol/g Hb

  
84378
  
10/08/2020
  
10/09/2020