Evaluate pancreatic disease.
Amylase is primarily produced in the pancreas and salivary glands. Isoenzymes may be used to determine the source of an elevated amylase concentration. Measurement of pancreatic amylase activity is of value in diagnosing pancreatitis and other pancreatic disorders which result in elevation of serum and urine amylase.
Immediately following collection, thoroughly mix sample by gently inverting 5 times
Gold serum separator (SST) tube
Immediately following collection, mix sample thoroughly by gently inverting 8 - 10 times to prevent clotting
Ambient (preferred ) - 7 days
Refrigerated - 30 days
Salivary amylase is inhibited by specific antibodies and the remaining pancreatic amylase is determined by an enzymatic, colorimetric method.
0 - 1 years: Not established
2 - 17 years: 12 – 40 Units/L
18 - 60 years: 14 – 55 Units/L
>60 years: 13 - 72 Units/L
Macroamylases are sometimes present in sera. These rare forms are probably complexes between ordinary amylase (usually S-type) and IgA, IgG, or other high molecular weight plasma proteins. These macroamylases cannot be filtered through the glomeruli of the kidney because of their large size (MW >200,000) and are thus retained in the plasma where their presence may increase amylase activity to some six- to eightfold over that observed in health. In macroamylasemia, amylase activity in the urine is lower than normal, since less amylase is cleared by the kidneys. No clinical symptoms are associated with this disorder; however, many of these cases have been detected following investigation of abdominal pain.