p40-TTF1 cocktail by IHC

Alphabetical Test listing

p40-TTF1 cocktail by IHC-12376 - Technical only, 12379 - Technical & interpretation

  
p40-TTF1 cocktail by IHC
  
12376 - Technical only, 12379 - Technical & interpretation
  
LAB12376
LAB12379
  
Cocktail
p40
TTF-1
  
  • All IHC stains will include a positive control tissue
  
  • This cocktail is intended to be used to assist in differentiating lung adenocarcinomas and lung squamous cell carcinomas. This TTF-1/P40 cocktail has a reported sensitivity of 77% and a specificity of 100% for lung adenocarcinomas. This TTF-1/P40coktail has a reported sensitivity of 93% and a specificity of 92% for lung squamous cell carcinomas
  • Other tumors reported to be positive for TTF-1 include neuroendocrine tumors (pulmonary and non-pulmonary origin), thyroid neoplasms, a subset of endometrial and cervical neoplasms, papillary nasopharyngeal carcinomas, and colorectal adenocarcinoma. Tumors which have been reported to rarely stain for TTF-1 in a subset of tumor cells include: cholangiocarcinoma, ovarian, gastric/esophageal, pancreatic ductal, urothelial, hepatocellular, salivary gland neoplasms including adenoid cystic carcinoma, and adrenal cortical carcinoma
  • Other tumors reported to be positive for the P40 clone used in this cocktail include bladder, breast, and prostate
  
Tissue
  

Submit a formalin-fixed, paraffin embedded tissue block

  

Formalin-fixed, paraffin embedded (FFPE) tissue block

  

FFPE tissue section mounted on a charged, unstained slide

  

Ambient (preferred)

  
  • Unlabeled/mislabeled block
  • Insufficient tissue
  • Slides broken beyond repair
  
AHL - Immunohistochemistry
  
Mo - Fr
  
1 - 2 days
  

Immunohistochemical staining and microscopic examination

  

If requested, an interpretive report will be provided

  

Specifications

  • TTF-1 reacts with a transcription factor expressed in thyroid (follicular cells), lung (type II cells, Clara cells), and diencephalon
  • P40 is one of 10 p63 isoforms which is highly specific for squamous differentiation

Staining pattern

  • Positive TTF-1 staining: Brown nuclear staining
  • Positive P40 staining: Red nuclear staining

  

References:

  1. Brown AF, et al. Tissue preserving antibody cocktails to differentiate primary squamous cell carcinoma, adenocarcinoma, and small cell carcinoma of lung. Arch Pathol Lab Med. 2013 Sep;137(9):1274-81.
  2. Russel D, et al. Dual immunostaining TTF-1 and p40 for subcategorizing of nonsmall cell carcinoma of the lung in cytologic and surgical specimens.
  
88342 - 1st stain
88341 - each additional stain
  
01/02/2018
  
10/19/2018
  
10/02/2020