CD20 by IHC

Alphabetical Test listing

CD20 by IHC-12376 - Technical only, 12379 - Technical & interpretation

CD20 by IHC
12376 - Technical only, 12379 - Technical & interpretation

All IHC stains will include a positive control tissue

  • CD20 is a very useful antibody which can identify most B cell lymphomas (approximately 95%)
  • CD20 also identifies Reed-Sternberg variants of nodular lymphocyte predominance Hodgkin’s disease (see section on immunohistochemistry panels)
  • Rare examples of CD20 positive T cell NHL have been reported3

Submit a formalin-fixed, paraffin-embedded tissue


Formalin-fixed, paraffin-embedded (FFPE) tissue block


FFPE tissue section mounted on a charged, unstained slide


Ambient (preferred)

  • Unlabeled/mislabeled block
  • Insufficient tissue
  • Slides broken beyond repair
AHL - Immunohistochemistry
Mo - Fr
1 - 2 days

Immunohistochemical staining and microscopic examination


If requested, an interpretive report will be provided



  • CD20 Reacts with an antibody found on the majority of B cells and is virtually B-cell specific

Staining pattern:

  • Cell surface membrane based and/or cytoplasmic pattern


  1. Cartun et. al.: Utilization of Monoclonal Antibody L26 in the Identification and Confirmation of B Cell Lymphomas. A Sensitive and Specific Marker Applicable to Formalin and B5 Fixed, Paraffin-embedded Tissues; American Journal of Clinical Pathology 129:415-421, 1987.
  2. Mason et. al.: Antibody L26 Recognizes and Intracellular Epitope on the B Cell Associated CD20 Antigen; American Journal of Clinical Pathology 135:1215-1222, 1990.
  3. Martinez et. al.: CD20 + T Cell Lymphoma; American Journal of Clinical Pathology 102:483-489, 1994.
88342 - 1st stain
88341 - each additional stain