Aneuploidy 4/10 by FISH
Aneuploidy 4/10 by FISH-4280H (blood) or 4280G (bone marrow)
LAB4280G - CYTOGENETIC BONE MARROW STUDIES
B-cell ALL
FISH
Fluorescence in situ hybridization
Trisomy 4
Trisomy 10
Detecting aneuploidy of chromosomes 4 and 10, recurring abnormalities in B-cell ALL and an indication of hyperdiploidy.
This test can be ordered individually or as part of the B-ALL FISH Panel. See the panel test for more details.
- Only one specimen tube is required to perform all ordered cytogenetics testing.
- The more testing requested, the closer to the optimal volume requirements is required.
- Immediately following collection, mix sample by inverting 8 - 10 times to prevent clotting.
Dk green Sodium heparin (Na hep), no gel
EDTA whole blood (a chromosome analysis study is not recommended for this specimen type)
Bone marrow
Collect bone marrow aspirate in a sterile Sodium heparin (Na hep) syringe and transfer to a sterile vial/tube
- or -
Collect bone marrow in sterile syringe (non-heparinized) and transfer to a Dk green Sodium heparin (Na hep), no gel
EDTA whole blood:
Lavender (EDTA), 4mL
Bone marrow:
Sterile vial/tube
Dk green Sodium heparin (Na hep), no gel
- AHL Cytogenetics oncology request
Ambient
- Improper label (unlabeled or mislabeled)
- Wrong container (anticoagulant or solution)
- Improper blood/anticoagulant ratio
- Delay in transport
- Improper storage
- Leaking container
Fluorescence in situ hybridization (FISH)
Normal or Abnormal with interpretation.
Gain (trisomy) of chromosomes 4 and 10 in combination is an indication of hyperdiploidy. Gain of chromosomes 4 and 10 is considered a favorable cytogenetics risk category in both pediatric and adult B-cell ALL according to NCCN guidelines.