Red blood cell membrane evaluation, blood

Alphabetical Test listing

Red blood cell membrane evaluation, blood-994

Test name:

Test number:

994

Excellian order number:

LAB994

Abbreviation:

MSO

Alternate names:

Band 3;EMA binding;Eosin-5-maleimide;Eosin hereditary spherocytosis;Hereditary pyropoikilocytosis;Osmotic Fragility;RBC membrane

Useful for:

Investigation of suspected red cell membrane disorders such as hereditary spherocytosis or hereditary pyropoikilocytosis

Specimen type:

EDTA whole blood

Collection container:

Lavender (EDTA), 4mL

Volume:

4.0 mL

Minimum volume:

2.0 mL

Collection instructions:

Submit a patient specimen, control specimen and 2 well-made peripheral blood smears (Wright stained or fixed in absolute methanol).

Allina facility special instructions:

Due to special requirements for collection, this test requires pathologist pre-approval by calling Client Services at (612) 863-4678.
If approved, collection must be scheduled in advance and is only performed at the Abbott-Northwestern, Mercy and United Hospital sites.
This test is performed by Mayo Clinic Laboratories (MCL). If it is more convenient for them, patients may go to a Mayo facility for specimen collection.

Processing instructions:

Refrigerate specimens immediately; do not spin.

Rubber band patient specimen and control vial together.

Patient specimen:

Immediately refrigerate specimen after draw at 0 - 4 °C
Do not freeze. Freezing causes sample lysis, and tests will not be performed on hemolyzed specimens.
Send specimen in original tube. Do not aliquot.
Rubber band patient specimen and control vial together. Control must accompany the patient sample at all times to ensure the reliability of testing results.
Be sure specimen and control are stored and transported together at refrigerate temperature, carefully following proper handling and shipping instructions.

Control specimen:

Draw a control specimen from a normal (healthy), unrelated, nonsmoking person at the same time as the patient.
Label clearly on outermost label normal control.
Immediately refrigerate specimen after draw at 0 - 4 °C.
Do not freeze. Freezing causes sample lysis, and tests will not be performed on hemolyzed specimens.
Send specimen in original tube. Do not aliquot.
Rubber band patient specimen and control vial together. Control must accompany the patient sample at all times to ensure the reliability of testing results.

Transport container:

Lavender (EDTA), 4mL

Transport and stability:

Refrigerated - 72 hours

Performing lab:

Mayo Clinic Laboratories (RBCME): R-NX

Days set up:

Mo - Sa

TAT:

4 - 7 days

Method:

HSEV: Consultative interpretation

FRAGO: Osmotic lysis

BND3: Flow cytometry

SMPB: Consultant review

Reference ranges:

<12 months:

Not established

≥ 12 months:

0.50 g/dL NaCl (unincubated): 3-53% hemolysis

0.60 g/dL NaCl (incubated): 14-74% hemolysis

0.65 g/dL NaCl (incubated): 4-40% hemolysis

0.75 g/dL NaCl (incubated): 1-11% hemolysis

An interpretive report will be provided.

CPT codes:

85557 - Osmotic fragility;88184 - Flow cytometry; first cell surface, cytoplasmic or nuclear marker x 1;85060 - Morphology review

Subject to medical necessity (Y/N):

Date created:

10/02/2019

Revised date:

01/21/2020