Myogenin by IHC

Alphabetical Test listing

Myogenin by IHC-12376 - Technical only, 12379 - Technical & Interpretation

  
Myogenin by IHC
  
12376 - Technical only, 12379 - Technical & Interpretation
  
LAB12376
LAB12379
  
  • All IHC stains will include a positive control tissue
  
  • Myogenin has been detected in the majority of routinely processed rhabdomyosarcomas and Wilms tumors
  • Myogenin is useful in a panel of immunostains (or frozen and/or paraffin sections) to evaluate small blue round cell tumors and poorly differentiated sarcomas
  • No immunoreactivity has been observed in Ewing's sarcoma/pPNET or in neuroblastomas
  • Myogenin will stain the nuclei of myoblasts from human fetal limbs, however no reactivity is observed in adult skeletal muscle1
  • Myogenin has not stained leiomyosarcoma, liposarcoma, alveolar soft part sarcoma, malignant fibrous histiocytoma, synovial sarcoma and malignant peripheral nerve sheath tumor in the small number of reported cases 2
  
Tissue
  

Submit a formalin-fixed, paraffin embedded tissue block

  

Formalin-fixed, paraffin embedded (FFPE) tissue block

  

FFPE tissue section mounted on a charged, unstained slide

  

Ambient (preferred)

  
  • Unlabeled/mislabeled block
  • Insufficient tissue
  • Slides broken beyond repair
  
AHL - Immunohistochemistry
  
Mo - Fr
  
1 - 2 days
  

Immunohistochemical staining and microscopic examination

  

If requested, an interpretive report will be provided

  

Specifications

  • Myogenin is a member of a family of myogenic regulatory proteins which includes MyoD, myf5 and MRF4
  • There genes encode transcription factors essential for muscle development
  • Expression of Myogenin is restricted to cells of skeletal muscle origin
  • Nuclear expression of myogenin is inversely related to the degree of cellular differentiation of rhabdomyosarcoma tumor cells1

 Staining pattern

  • Nuclear staining only
  • Regional staining pattern is common

References

  1. Wang NP, et. al.: Expression of Myogenic Regulatory Proteins (Myogenin and MyoD1) in Small blue Round Cell Tumors of Childhood. Amer J. Pathol., 147(6):1799-1810, 1995.
  2. Tallini G, et. al.: Myogenic Regulatory Protein Expression in Adult Soft Tissue Sarcomas. Amer J. Pathol, 144(4):693-701, 1994.
  
88342 - 1st stain
88341 - each additional stain
  
07/18/2017
  
10/19/2018
  
10/02/2020