GCDFP-15 by IHC

Alphabetical Test listing

GCDFP-15 by IHC-12376 - Technical only, 12379 - Technical & interpretation

  
GCDFP-15 by IHC
  
12376 - Technical only, 12379 - Technical & interpretation
  
LAB12376
LAB12379
  
AP-15
BRST-2
Gross cystic disease fluid protein 15
  
  • Useful in identifying tumors of breast origin, although negative GCDFP-15 stains do not exclude possibility of breast carcinoma1
  • Approximately 77% of infiltrating ductal and 53% of invasive lobular carcinomas are GCDFP-15 positive
  • Useful in discriminating primary ovarian tumors from breast cancers metastatic to ovary2
  • Useful in battery with S-100, CEA, and ER in discriminating primary lung carcinomas from breast carcinoma metastatic to lung3
  
Tissue
  

Submit a formalin-fixed, paraffin-embedded tissue

  

Formalin-fixed, paraffin-embedded (FFPE) tissue block

  

FFPE tissue section mounted on a charged, unstained slide

  

Ambient (preferred)

  
  • Unlabeled/mislabeled block
  • Insufficient tissue
  • Slides broken beyond repair
  
AHL - Immunohistochemistry
  
Mo - Fr
  
1 - 2 days
  

Immunohistochemical staining and microscopic examination

  

If requested, an interpretive report will be provided

  

Specifications

  • GCDFP-15 is a protein identified in gross cystic fluid of breast
  • GCDFP-15 is expressed by some breast carcinomas (particularly those with apocrine differentiation), apocrine and eccrine glands, minor salivary glands, bronchial glands, and related tumors
  • GCDFP-15 is expressed in virtually 100% of cases of Paget’s disease of skin (extramammary)
  • GCDFP-15 expression has also been identified in prostate and rarely in ovarian, renal, and bladder carcinomas

Staining pattern

  • “Mosaic”*, cytoplasmic based staining.
    * mosaic pattern refers to very focal or patchy reactivity

References

  1. Wick, et. al: Gross Cystic Disease Fluid Protein – 15 as a Marker for Breast Cancer; Human Pathol 20:281-287, 1989.
  2. Monteagudo, et. al: Value of Gross Cystic Disease Fluid Protein-15 in Distinguishing Metastatic Breast Carcinomas Among Poorly Differentiated Neoplasm Involving the Ovary; Human Pathol 22:368-372, 1991.
  3. Raab, et. al: Adenocarcinoma in the Lung in Patients with Breast Cancer; American Journal of Clinical Pathol 100:27-35, 1993.
  
88342 - 1st stain
88341 - each additional stain
  
07/03/2017
  
10/18/2018
  
10/02/2020