Factor VIII antigen

Alphabetical Test listing

Factor VIII antigen-994

Test name:

Factor VIII antigen

Test number:

994

Excellian order number:

LAB994

Abbreviation:

MSO

Alternate names:

Factor 8 Ag
Factor 8 antigen
FVIII quantitation (non-functional)

Useful for:

Quantitate the amount of factor VIII protein; not a measure of von Willebrand factor antigen (previously called FVIII-related antigen)

Specimen type:

Sodium citrate (Na cit) plasma

Collection container:

Lt blue Sodium citrate (NaCit) - 2.7mL x 2

Volume:

2.0 mL

Minimum volume:

1.0 mL

Collection instructions:

Do not over or under fill tube as the ratio of anticoagulant to whole blood is critical

Coag – tube fill guidelines

Immediatley following collection, mix sample thoroughly by gentle inverting 8 - 10 times, to prevent clotting

Processing instructions:

Centrifuge for 10 minutes and carefully remove 2/3 of the plasma using a plastic transfer pipette, being careful not to disturb the cells.
Deliver to a plastic transport tube, cap, and recentifuge for 10 minutes.
Use a second plastic pipette to remove plasma, staying clear of the platelets at the bottom of the tube.
Transfer the plasma into a Screw-cap polypropylene frozen transport vial/tube - 4mL (LabCorp), labelled as Na cit plasma
Freeze immediately.

Transport container:

Screw-cap polypropylene frozen transport vial/tube - 4mL (LabCorp)

Transport and stability:

Frozen (-20) - 1 month

Frozen (-70) - 6 months

Three (3) freeze/thaw cycles are acceptable

Refrigerated - 8 hours

Ambient - 4 hours

Performing lab:

LabCorp Burlington (500196): R-NX

Days set up:

TAT:

3 - 7 days

Method:

Enzyme-linked immunosorbent assay (ELISA)

Reference ranges:

50 - 160%

CPT codes:

83520

LOINC code:

Result 38521-1

Date created:

10/11/2019

Revised date:

02/17/2021

Review date:

06/03/2020