CD61 by IHC

Alphabetical Test listing

CD61 by IHC-12376 - Technical only, 12379 - Technical & interpretation

  
CD61 by IHC
  
12376 - Technical only, 12379 - Technical & interpretation
  
LAB12376
LAB12379
  
Platelet glycoprotein IIIa
  

All IHC stains will include a positive control tissue

  
  • CD61 is the most sensitive and most specific of the platelet glycoprotein antibodies
  • CD61 is the earliest glycoprotein to be expressed during normal megakaryocyte maturation
  • 80% of AML-M7 react with CD61; 20% are more immature and lack CD61 (diagnosed by the presence of platelet peroxidase by EM)
  • The platelets from patients with Glanzmann's thrombasthenia lack both glycoproteins IIb and IIIa
  • CD61 can identify small platelet clumps (microthrombi) and may be of use in renal transplantation biopsies as well as studies of ischemic heart disease, inflammatory/ ischemic bowel disease, and placental pathology, etc.
  • No cross reactivity with a variety of formalin-fixed paraffin-embedded normal and neoplastic tissues have been reported
  
Tissue
  

Submit a formalin-fixed, paraffin-embedded tissue

  

Formalin-fixed, paraffin-embedded (FFPE) tissue block

  

FFPE tissue section mounted on a charged, unstained slide

  

Ambient (preferred)

  
  • Unlabeled/mislabeled block
  • Insufficient tissue
  • Slides broken beyond repair
  
AHL - Immunohistochemistry
  
Mo - Fr
  
1 - 2 days
  

Immunohistochemical staining and microscopic examination

  

If requested, an interpretive report will be provided

  

Specifications

  • CD61 is the integrin B-3-chain of the vitronectin receptor. This protein is found on platelets and megakaryocytes, and can associate with either glycoprotein IIb or vitronectin receptor ?-chain (CD51)

Staining pattern

  • Cytoplasm of megakaryocytes, surface of platelets
  • Well preserved in formalin
  • Loss of antigenicity has been reported with mercuric fixatives

References

  1. Gatter KC, et al. The immunohistological detection of platelets, megakaryocytes and thrombi in routinely processed specimens. Histopathology 1988; 13:257-67.
  2. Erber WN, et al. Detection of cells of megakaryocyte lineage in hematological malignancies by immuno-alkaline phosphatase labeling cell smears with a panel of monoclonal antibodies. British Journal of Hematology 1987; 65:87-94.
  3. Cheson BD, et al. Report of the National Cancer Institute sponsored workshop on definitions of diagnosis and response in acute myeloid leukemia. Journal of Clinical Oncology 1990; 8(5):813-9.
  
88342 - 1st stain
88341 - each additional stain
  
06/16/2017
  
10/18/2018
  
10/02/2020