SOX11 by IHC

Alphabetical Test listing

SOX11 by IHC-12376 - Technical only, 12379 - Technical & interpretation

  
SOX11 by IHC
  
12376 - Technical only, 12379 - Technical & interpretation
  
LAB12376
LAB12379
  
  • All IHC stains will include a positive control tissue
  
  • Most (90%) mantle cell lymphomas are SOX11 positive (even when t(11;14) translocation and cyclin D1 are not detected) and most mimics are SOX11 negative
  • Sox11 identifies the 2% of mantle cell lymphomas that lack Bcl-1/cyclinD1/CCND1 IP staining and standard t(11;14) FISH
  • 10% of mantle cell lymphomas are SOX11 negative, and these cases tend to present more frequently with non-nodal leukemic disease and have a more indolent course
  • SOX11 should be ordered with Bcl-1/cyclin D1
  • This antibody can be used to differentiate blastoid MCL from CD5-positive diffuse large B-cell lymphoma (DLBCL). However, note that rare (2 of 43 in one series) DLBCL showed weak nuclear SOX-11 expression
  • SOX11 expression occasionally can be seen in occasional examples of other lymphoid malignancies, including Burkitt lymphoma (BL), acute lymphoblastic leukemia/lymphoma (ALL/LBL), T-cell prolymphocytic leukemia, marginal zone lymphoma, and anaplastic large cell lymphoma (ALK-negative)
  • CLL/SLL may have proliferation centers that express bcl-1/cyclin D1, but these cells do not have t(11;14) and lack SOX11
  • SOX11 expression has also been shown to be a favorable prognostic marker in glioblastoma.
  
Tissue
  

Submit a formalin-fixed, paraffin embedded tissue block

  

Formalin-fixed, paraffin embedded (FFPE) tissue block

  

FFPE tissue section mounted on a charged, unstained slide

  

Ambient (preferred)

  
  • Unlabeled/mislabeled block
  • Insufficient tissue
  • Slides broken beyond repair
  
AHL - Immunohistochemistry
  
Mo - Fr
  
1 - 2 days
  

Immunohistochemical staining and microscopic examination

  

If requested, an interpretive report will be provided

  

Specifications

  • SOX11 antibody (SRY (Sex Determining Region Y)-Box 11) is a member of the SOX family of transcription factors, involved in the regulation of embryonic development and in the determination of cell fate
  • SOX11 is expressed in most mantle cell lymphomas, including cyclin D1 negative cases

 Staining pattern

  • Nuclear staining

References

  1. Soldini D et al: Assessment of SOX11 Expression in Routine Lymphoma Tissue Sections; Characterization of New Monoclonal Antibodies for Diagnosis of Mantle Cell Lymphoma. Am J Surg Pathol 2014;38:86-93.
  2. Vose JM: Mantle cell lymphoma: 2015 update on diagnosis, risk-stratification, and clinical management. Am. J. Hematol. 90:740-745, 2015.
  3. Nakashima MO et al: Utility and Diagnostic Pitfalls of SOX11 Monoclonal Antibodies in Mantle Cell Lymphoma and Other Lymphoproliferative Disorders. Appl Immunohistochem Mol Morphol 2014;22:720-727.
  4. Sander B et al: Mantle cell lymphoma - a spectrum from indolent to aggressive disease. Virchows Archives DOI 10.1007/s00428-015-1840-6.
  
88342 - 1st stain
88341 - each additional stain
  
09/06/2017
  
10/17/2018
  
10/02/2020