Epithelial membrane antigen by IHC

Alphabetical Test listing

Epithelial membrane antigen by IHC-12376 - Technical only, 12379 - Technical & interpretation

  
Epithelial membrane antigen by IHC
  
12376 - Technical only, 12379 - Technical & interpretation
  
LAB12376
LAB12379
  
EMA
  

All IHC stains will include a positive control tissue

  
  • Breast and skin adnexa are usually strongly EMA positive
  • Variety of other carcinomas that are EMA positive include carcinomas of endometrium, kidney, thyroid, stomach, pancreas, lung, colon, ovary, prostate, cervix
  • EMA positivity may be seen in mesotheliomas; transitional and squamous cell carcinomas may show variable reactivity
  • EMA also labels plasma cells, NHL with plasmacytic differentiation, and anaplastic large cells lymphomas (Ki-1 lymphomas)
  • Hepatocellular carcinomas, adrenocortical carcinomas, embryonal carcinomas and basal cell carcinomas are (usually) negative for EMA

EMA positivity in NHL and HD:

B cell NHL 5%
Plasma cell 80%
T cell NHL 20%
Anaplastic (Ki-1) 80%
L & H nodular HD 60%
Classical HD 20%
  
Tissue
  

Submit a formalin-fixed, paraffin-embedded tissue

  

Formalin-fixed, paraffin-embedded (FFPE) tissue block

  

FFPE tissue section mounted on a charged, unstained slide

  

Ambient (preferred)

  
  • Unlabeled/mislabeled block
  • Insufficient tissue
  • Slides broken beyond repair
  
AHL - Immunohistochemistry
  
Mo - Fr
  
1 - 2 days
  

Immunohistochemical staining and microscopic examination

  

If requested, an interpretive report will be provided

  

Specifications

  • EMA is a glycoprotein isolated from human milk fat globule membranes
  • EMA is present in many types of epithelia
  • EMA can also be identified in mesenchymal and hematopoietic cells

Staining pattern

  • Cytoplasmic based staining and +/- cell surface membrane staining

  
88342 - 1st stain
88341 - each additional stain
  
06/21/2017
  
10/17/2018
  
10/02/2020