Epithelial membrane antigen by IHC

Alphabetical Test listing

Epithelial membrane antigen by IHC-12376 - Technical only, 12379 - Technical & interpretation

Epithelial membrane antigen by IHC
12376 - Technical only, 12379 - Technical & interpretation

All IHC stains will include a positive control tissue

  • Breast and skin adnexa are usually strongly EMA positive
  • Variety of other carcinomas that are EMA positive include carcinomas of endometrium, kidney, thyroid, stomach, pancreas, lung, colon, ovary, prostate, cervix
  • EMA positivity may be seen in mesotheliomas; transitional and squamous cell carcinomas may show variable reactivity
  • EMA also labels plasma cells, NHL with plasmacytic differentiation, and anaplastic large cells lymphomas (Ki-1 lymphomas)
  • Hepatocellular carcinomas, adrenocortical carcinomas, embryonal carcinomas and basal cell carcinomas are (usually) negative for EMA

EMA positivity in NHL and HD:

B cell NHL 5%
Plasma cell 80%
T cell NHL 20%
Anaplastic (Ki-1) 80%
L & H nodular HD 60%
Classical HD 20%

Submit a formalin-fixed, paraffin-embedded tissue


Formalin-fixed, paraffin-embedded (FFPE) tissue block


FFPE tissue section mounted on a charged, unstained slide


Ambient (preferred)

  • Unlabeled/mislabeled block
  • Insufficient tissue
  • Slides broken beyond repair
AHL - Immunohistochemistry
Mo - Fr
1 - 2 days

Immunohistochemical staining and microscopic examination


If requested, an interpretive report will be provided



  • EMA is a glycoprotein isolated from human milk fat globule membranes
  • EMA is present in many types of epithelia
  • EMA can also be identified in mesenchymal and hematopoietic cells

Staining pattern

  • Cytoplasmic based staining and +/- cell surface membrane staining

88342 - 1st stain
88341 - each additional stain