Lt green plasma separator (PST)

1. Give patient 50 gm glucose dose
2. Draw 1 hour after dose
3. Immediately following collection, mix sample thoroughly by gentle inverting 8 - 10 times to prevent clotting

Spin within two (2) hours of sample collection

Lt green plasma separator (PST)

Gold serum separator (SST) tube

Grey Sodium flouride (NaFl)

Lavender (EDTA), 4mL

Gold:

1. Allow sample to clot for a minimum of 30 minutes
2. Spin within two (2) hours of sample collection

Lavender:

1. Spin
2. Transfer the plasma to an AHL False Bottom Plasma/Serum Transport Tube labeled as the appropriate plasma type, within two (2) hours of sample collection

Grey:

If a grey top tube will not reach the Central Laboratory within 24 hours of collection, it should be spun down, and the plasma transferred to an AHL False Bottom Plasma/Serum Transport Tube labeled as NaFl plasma. 

Additional testing cannot be added on to this sample.

Gold serum separator (SST) tube

Grey Sodium flouride (NaFl) or

False bottom plasma/serum transport vial/tube (AHL)

Architect:

Serum/plasma:

Refrigerated  - 7 days

NaFl whole blood:

Refrigerated - 24 hours

Roche:

Serum/plasma:

Refrigerated - 3 days

Ambient - 8 hours

NaFl whole blood:

Ambient - 3 days

• Improper labels (unlabeled or mislabeled)
• Hemolysis (some procedures)
• Improper anticoagulant or ratio
• Delay in transport
• Improper storage temperature affecting results
• Inappropriate timing of collection
• Improper container
• Leaking container resulting in compromised specimen
• Quantity not sufficient (QNS)

Hexokinase

Architect:

< 140 mg/dL

Roche:

70 - 139 mg/dL

A glucose result of >140 indicates the need for more extensive testing (i.e. 3 hr Gestational tolerance)