Adenovirus is an important cause of morbidity and mortality in the transplant setting, causing pneumonia, hemorrhagic cystitis, hepatitis, encephalitis, pancreatitis, enteritis, and disseminated disease with the mortality rate reaching 60% in some especially high risk situations such as pediatric hematopoietic stem cell transplantation. Since proper management is dependent upon early diagnosis, quantitative adenovirus DNA PCR is useful for detecting the virus, tracking the course of infection, and monitoring response to treatment.
Treatment of adenovirus in immunocompromised patients presents challenges, including drug toxicity, delayed onset of disease after discontinuing therapy, and emergence of mutations that may affect the ability of diagnostic assays to detect them efficiently.
To avoid delays in turnaround time when requesting multiple tests on frozen samples, submit a separate frozen aliquot for each test requested.
Frozen (preferred) - 30 days
Refrigerated - 14 days
Ambient - 96 hours
Real-time polymerase chain reaction (RT-PCR)