Estrogen receptor by IHC

Alphabetical Test listing

Estrogen receptor by IHC-12376 - Technical only, 12379 - Technical & interpretation

  
Estrogen receptor by IHC
  
12376 - Technical only, 12379 - Technical & interpretation
  
LAB12376
LAB12379
  
ER
  

All IHC stains will include a positive control tissue

  
  • ER is used to determine if a tumor expresses estrogen receptor (i.e. for classification purposes)
  • ER positivity is used to determine if a patient would be a candidate for anti-estrogen therapy
  • Note: ER positivity may be seen in 5-10% of lung, nonsmall cell carcinomas (up to 18% with our previous antibody 1D5); staining for ER in lung tumors is usually focal and variable in intensity 7; In the context of an ER-positive lung neoplasm, strong and extensive TTF-1 immunoreactivity can be regarded as strong supportive evidence for a primary bronchogenic adenocarcinoma
  
Tissue
  

Submit a formalin-fixed, paraffin-embedded tissue

  

Formalin-fixed, paraffin-embedded (FFPE) tissue block

  

FFPE tissue section mounted on a charged, unstained slide

  

Ambient (preferred)

  
  • Unlabeled/mislabeled block
  • Insufficient tissue
  • Slides broken beyond repair
  
AHL - Immunohistochemistry
  
Mo - Fr
  
1 - 2 days
  

Immunohistochemical staining and microscopic examination

  

If requested, an interpretive report will be provided

  

Specifications

  • ER is a nuclear protein that binds estrogen hormone
  • ER expression is seen in a variety of tumors from breast or gynecologic origin
  • ER expression can also be seen in tumors of lung, stomach, thyroid, and very rarely colorectal origin

 
Staining pattern

  • Nuclear staining

References

  1. Deamant FD, et al: Estrogen receptor immunohistochemistry as a predictor of site of origin in metastatic breast cancer. Appl Immunohistochem 1(3): 188-192, 1993.
  2. Esteban JM, et al: Biologic significance of quantitative estrogen receptor immunohistochemical assay by image analysis in breast cancer. Am J Clin Pathol 1994: 102:158-162.
  3. Aziz DC: Quantitation of estrogen and progesterone receptors by immunocytochemical and image analyses. Am J Clin Pathol 1992; 98:105-111.
  4. Wilbur DC et al: Estrogen and progesterone receptor detection in archival formalin-fixed, paraffin-embedded tissue from breast carcinoma: A comparison of immunohistochemistry with the dextran-coated charcoal assay. Mod Pathol, 5(1), 1992.
  5. Esteban JM et al: Improvement of the quantification of estrogen and progesterone receptors in paraffin-embedded tumors by image analysis. Am J Clin Pathol, 1993; 99:32-38.
  6. Taylor CR: Paraffin section immunocytochemistry for estrogen receptor; the time has come. Cancer 77(12), 1996.
  7. Lau SK et al: Immunohistochemical expression of estrogen receptor in pulmonary adenocarcinoma. Appl Immunohistochem Mol Morphol. 2006 Mar; 14 (1): 83-7.
  
88342 - 1st stain
88341 - each additional stain
  
06/21/2017
  
10/17/2018
  
10/02/2020