MDS panel by FISH

Alphabetical Test listing

MDS panel by FISH-Order individual components; 4280H (blood) or 4280G (bone marrow)

  
MDS panel by FISH
  
Order individual components; 4280H (blood) or 4280G (bone marrow)
  
4280H - CYTOGENETIC LEUKEMIC BLOOD STUDIES
4280G - CYTOGENETIC BONE MARROW STUDIES
  
Deletion 5q31
Deletion 7q31
Deletion 20q
FISH
Fluorescence In Situ Hybridization
MDS
MLL
Monosomy 5
Monosomy 7
Trisomy 8
Myelodysplastic syndrome
t(11q23;?)
5q-
-5
7q-
-7
+8
  
  • 5Q-
  • 7Q-
  • FGFR1 (+8)
  • 20Q-
  • MLL
  

The MDS FISH panel is useful for detecting the recurrent FISH abnormalities associated with MDS NCCN guidelines

  
Sodium heparin (Na hep) whole blood
  
  
5 mL
  
2.0 mL
  
  • Only one specimen tube is required to perform all ordered cytogenetics testing.
  • The more testing ordered, the closer to the optimal volume requirement is preferred.
  

Dk green Sodium heparin (Na hep), no gel

  
Bone marrow
EDTA whole blood (chromosome analysis study is not recommended for this specimen type)
  

Whole blood: (not recommended)

Lavender (EDTA), 4mL

  
Bone marrow: 10 mL (minimum 5 mL)
  

Bone marrow

Collect bone marrow aspirate in a sterile Sodium heparin (Na hep) syringe and transfer to a sterile vial/tube

  

- or -

Collect bone marrow in sterile syringe (non-heparinized) and transfer to a Dk green Sodium heparin (Na hep), no gel

  

Bone marrow:

Sterile vial/tube

Dk green Sodium heparin (Na hep), no gel

 

EDTA whole blood:

Lavender (EDTA), 4mL

  
  • AHL Cytogenetics oncology requisition
  

Ambient

  
Pellets - 2 years
  
  • Improper label (unlabeled or mislabeled)
  • Wrong container (anticoagulant or solution)
  • Improper blood/anticoagulant ratio
  • Delay in transport
  • Improper storage
  • Leaking container
  
AHL - Cytogenetics: X
  
Mo, We, Fr
  
5 - 7 days
  

Fluorescence in situ hybridization (FISH)

  

Normal or Abnormal with interpretation.

  • Deletion of 11q (including MLL) is classified as a very good cytogenetic prognostic indicator in MDS.
  • Deletion of 5q- and 20q- are classified as good cytogenetic prognostic indicators in MDS.
  • 7q- and +8 are classified as intermediate cytogenetic prognostic indicators in MDS.
  • -7 is classified as a poor cytogenetic prognostic indicator in MDS.
  
88271 x 11
88275 x 5
  
Yes
  
  
03/26/2021