T & B cell screen

Alphabetical Test listing

T & B cell screen-12420

  
T & B cell screen
  
12420
  
LAB12420
  
T&B
  
Flow
Flow Cytometry
B cell
Full B panel
Leukemia
Lymphoma
Lymphocyte phenotype
Lymphocyte screen
T cell
  
Tubes:
B1
T1 (10C)

Markers:
CD2
CD3
CD4
CD5
CD7
CD8
CD10
CD19
CD20
CD45
CD56
Kappa
Lambda
TCRγδ (TCR gamma delta)
TRBC1
  

Screen for aberrant T cells, light chain-restricted B cells and excess NK cells

  
EDTA whole blood
  
  
4 mL
  
1.0 mL
  

Lavender (EDTA), 4mL

  
Body fluid
Bone marrow
Cerebrospinal fluid (CSF)
Tissue
  
Varies; see Alternate collection instructions
  

Body fluid

  • Collect 20 mL (minimum 5 mL) of body fluid and place in a Falcon vial/tube - 15mL
    • Do not add anticoagulant or holding media
    • The volume required is dependent upon the white blood cell (WBC) count. Smaller volumes are sufficient if the cell count is high

Bone marrow

  • Collect 3 mL (minimum 1 mL) in a sodium heparinized (Na hep) syringe
    • Specimens collected in EDTA are acceptable, but not preferred
  • Transfer to an anticoagulant free sterile tube
  • If unable to collect a bone marrow aspirate, a bone marrow core placed in RPMI with Hepes, Pen Strep and FBS is acceptable

Cerebrospinal fluid (CSF)

  • Collect 5 mL (minimum 1 mL) in a sterile vial/container.
    • Do NOT add any anticoagulant or holding media
    • The volume required is dependent upon the white blood cell (WBC) count. Smaller volumes are sufficient if the cell count is high

Tissue

  • Immediately following collection, place the fine needle aspirate (FNA) or tissue in RPMI with Hepes/Pen strep/FBS -15 mL
  

Body fluid

Falcon vial/tube - 15mL

Bone marrow

Bone marrow transport vial/tube

Cerebrospinal fluid (CSF)

Sterile vial/container

Tissue

RPMI with Hepes/Pen strep/FBS -15 mL

  

Manual request clients: 

Complete the "Brief clinical information" and "What do you want answered by Flow?" areas on the request form. Include previous Allina case number, if applicable.

  

EDTA whole blood

Ambient

Body fluid

Refrigerated

Bone marrow

Ambient

Cerebrospinal fluid (CSF)

Refrigerated

Tissue

Refrigerated

  
14 days
  
  • Improper label (unlabeled or mislabeled)
  • Wrong container (anticoagulant or solution)
    • Tissues submitted in saline or B5 fixative are not acceptable and will be rejected
  • Improper blood/anticoagulant ratio
  • Delay in transport
  • Improper storage
  • Inappropriate timing of collection
  • Interfering substances
  • Leaking container
  
AHL - Flow Cytometry: F
  
Mo - Fr
  
2 days
  

Flow cytometry

  

Tubes and Markers:

Tubes: B1 and T1 (10C)

Markers: CD2, CD3, CD4, CD5, CD7, CD8, CD10, CD19, CD20, CD45, CD56, Kappa, Lambda, TCRγδ (TCR gamma delta) and TRBC1

  
88184
88185 x 14
  
08/05/2021
  
08/31/2021
  
08/31/2021