Epstein Barr Virus in situ hybridization (ISH)

Alphabetical Test listing

Epstein Barr Virus in situ hybridization (ISH)-12376 - Technical only, 12379 - Technical & interpretation

  
Epstein Barr Virus in situ hybridization (ISH)
  
12376 - Technical only, 12379 - Technical & interpretation
  
LAB12376
LAB12379
  
EBV
EBV in situ hybridization
EBV ISH
  
  • All ISH stains will include a positive control tissue
  
  • EBV is a member of Herpes virus family, and is the causative agent in infectious mononucleosis EBV is associated with some cases of Hodgkin's disease, Burkitt's lymphoma, peripheral T-cell lymphoma, gastric carcinoma and nasopharyngeal carcinoma EBV is also associated with post-transplant lymphoproliferative disorder (PTIPD)
  • The EBV in situ test is much more sensitive than the EBV IHC test
  
Tissue
  

Submit a formalin-fixed, paraffin embedded tissue block

  

Formalin-fixed, paraffin embedded (FFPE) tissue block

  

FFPE tissue section mounted on a charged, unstained slide

  

Ambient (preferred)

  
  • Unlabeled/mislabeled block
  • Insufficient tissue
  • Slides broken beyond repair
  
AHL - Immunohistochemistry
  
Mo - Fr
  
1 - 2 days
  

In situ hybridization and microscopic examination

  

If requested, an interpretive report will be provided

  

Probe

EBV (EBER; Epstein-Barr Virus Early RNA)+R40 RNA probe

Specifications

Recognizes early RNA transcript of EBV infection The RNA transcript accumulates in nuclei of actively infected cells

Staining pattern

  • Nuclear

References

1. Ambinder RF et al: Epstein-Barr-encoded RNA in situ hybridization: diagnostic applications. Human Pathol 25:602-605, 1994.

2. Randhawa P.S et al: Recurrent Epstein-Barr virus-associated lesions in organ transplant recipients. Hum Pathol. Feb;27(2):157-64. 1996.

 

 

  
88365 - 1st stain
88364 - each additional stain
  
10/30/2017
  
10/17/2018
  
10/02/2020