Vascular endothelial growth factor, alpha

Alphabetical Test listing

Vascular endothelial growth factor, alpha-994

This sendout test is restricted and cannot be ordered, collected or sent by Allina Health unless an exception has been approved in advance.   

Rare exceptions for approved clinical indications require pathologist review through Client Services before placing an order or collecting a specimen.

Orders or specimens received without pre-approval will be cancelled.  

  
Vascular endothelial growth factor, alpha
  
994
  
LAB994
  
MSO
  
VEGF-A
VEGF alpha
Cytokine
  

 

 

 

  

Elevated circulating concentrations of vascular endothelial growth factor (VEGF) may be observed in a variety of disease states, especially those associated with  angiogenesis. Elevated concentrations of VEGF must be interpreted within the clinical context of the patient.

For patients with a diagnosis of POEMS syndrome.

  
EDTA plasma
  
  
0.5 mL
  
0.3 mL
  

Immediatley following collection, mix sample thoroughly by gentle inverting 8 - 10 times, to prevent clotting, and then place the tube on wet ice

  
  1. Centrifuge at 1500 x g for 10 minutes
  2. Transfer plasma to a Screw cap transfer vial/tube (Mayo), labelled as EDTA plasma
  3. Freeze specimen within 30 minutes
  
  

Frozen (strict) - 21 days

Refrigerated - 24 hours

  
14 days
  
  • Gross lipemia
  • Gross icterus
  
Mayo Clinic Laboratories (VEGF): R-NX
  
Th
  
2 - 8 days
  

The vascular endothelial growth factor (VEGF) cytokine assay measures human cytokines in a 96-well spotted plate. The assay employs a sandwich immunoassay format where capture antibodies are coated on a single spot on the bottom of each well. Diluted samples, calibrators, and controls are added and to the plate. If present, VEGF will bind to the capture antibodies. After incubation, a solution containing detection antibodies conjugated with electrochemiluminescent labels is added. After a final incubation, a buffer is added that creates the appropriate chemical environment for electrochemiluminescence. The plate is then read on the Sector Imager 2400. The machine applies a voltage that causes bound labels to emit measurable light. The Sector Imager 2400 measures the intensity of emitted light and correlates it to a set of standards of known quantity via a 4-point logistics curve fitting method.(Package Insert: Human VEGF V-plex, Mesoscale Discovery, Rockville, MD 20850 USA, 18095-v2-2014 Jan)

  

≤96.2 pg/mL

  
This test can only be ordered with pathologist pre-approval
  
83520
  
34694-0
  
09/28/2011
  
02/19/2020
  
02/20/2020