Lt blue Sodium citrate (NaCit) - 2.7mL

If the patient has a hematocrit >55, a specially prepared Lt blue Sodium citrate (NaCit) tube must be used in place of the standard Lt blue Sodium citrate (NaCit) tube.

Hematocrit-Anticoagulant adjustments

Do not over or under fill the tube as the ratio of anticoagulant to whole blood is critical for accurate results

Coag – tube fill guideline

Immediatley following collection, mix sample thoroughly by gentle inverting 8 - 10 times, to prevent clotting

1. Process Platelet Poor Plasma (P.P.P).

   • Coag – how to prepare a specimen for special coagulation testing

2. Transfer plasma into a Transfer vial/tube with cap - 12mL (LabCorp) and apply a spot label "NaCit Plasma P.P.P"

Transfer vial/tube with cap - 12mL (LabCorp)

Lavender (EDTA), 4mL

OR

Lt green plasma separator  (PST)

Immediately following collection, thoroughly mix sample by gently inverting 5 times

1. Spin within 2 hours of sample collection

2. Transfer plasma to a Transfer vial/tube with cap - 12mL (LabCorp), labelled as plasma

Transfer vial/tube with cap - 12mL (LabCorp)

Ambient (preferred) - 7 days

Refrigerated - 14 days

Frozen - 14 days

Freeze/thaw cycles - stable x3

• Hemolyzed specimens
• Patient on heparin > 1.0 IU/mL
• Specimen exceeds standard testing time
• Unlabeled or mislabeled specimen
• Clotted specimen
• Over/under filled tube (incorrect volume)

Liquid chromatography/tandem mass spectrometry (LC/MS-MS)