Lt blue Sodium citrate (Na Cit) - 2.7mL 

If the patient has a hematocrit >55, a specially prepared Lt blue Sodium citrate (NaCit) tube must be used in place of the standard Lt blue Sodium citrate (NaCit) tube.

Hematocrit-Anticoagulant adjustments

• Do not over or under fill tube as the ratio of anticoagulant to whole blood is critical

Coag – tube fill guidelines

• Immediately following collection, mix by inverting 8 - 10 times to prevent clotting

Microcentrifuge vial/tube in a Snap cap conical vial/tube and cap (Beaker sites)

Coagulation specimen transport vial/tube  (all other sites)

STAT requests only:

Send whole blood sample in original collection container with STAT courier.

Must be tested within 4 hours of collection.

Lt blue Sodium citrate (Na Cit) - 2.7mL 

Routine:

Frozen (strict)

Refrigerated - NO

Ambient - NO

Stat:

Ambient - 4 hours

• Samples collected in Greiner tubes
• Improper anticoagulant
• Improperly (over or under) filled tube
• Hemolysis
• Patient on heparin > 1.0 IU/mL
• Exceeds standard testing time
• Incorrectly labeled or mislabeled
• Clotted specimen
• Plasma samples not completely frozen

Clot based assay

<16 sec

Prolongation of the thrombin time indicates:

• An abnormality of fibrinogen
  • Qualitative; dysfibrinogenemia
  • Quantitative congenital: hypofibrinogenemia or afibrinogenemia
  • Quantitative acquired: DIC, fibrinolysis, liver disease
• The presence of antithrombins
  • Therapeutic: heparin, hirudin, argatroban